Abbe’s diffraction formula for lateral (i.e. XY) resolution is: d= λ/2 NA. Where λ is the wavelength of light used to image a specimen. If using a green light of 514 nm and an oil immersion objective with an NA of 1.45, then the (theoretical) limit of resolution will be 177 nm. Abbe’s diffraction formula for axial (i.e. Z) resolution is:
The resolution of a microscope can be defined as the smallest distance at which two small objects can still be seen as separate objects. This lecture discusses various criteria for resolution, the factors that influence resolution in the lateral and axial planes, and how to sample an image adequately using a camera or confocal microscope, such
Aug 12, 2008· How fast the control is able to do this will depend on a number of internal factors that are beyond the scope of this text. The best solution is to select a micro-milling machine with proven control technology from a respected and well-known company. In this case, support will become a natural spin-off providing years of excellent support.
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A watchmakers lathe is a good starting point owing to the precision of the slides and spindles, but it lacks the z-axis. In more recent years kits became available to convert Chinese-made watchmakers lathes into small vertical milling machines, but the milling table on them is arranged in a conventional way.
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Precision and accuracy of stable isotope signals in otoliths of pen-reared cod (Gadus morhua) when sampled with a high-resolution micromill. Marine Biology. 2004; 144:1039–1049. doi:
An ultrahigh vacuum focused ion beam micromilling apparatus and process areisclosed. Additionally, a durable data storage medium using the micromilling process is disclosed, the durable data storage medium capable of storing, e.g., digital or alphanumeric characters as
Systems and methods for continuously casting Al—Mg alloy sheet or plate product having a high amount of magnesium are disclosed. The Al—Mg products have 4 or 6 to 8 or 10 wt. % Mg and are resistant to both stress corrosion cracking and intergranular corrosion.
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A real dynamic population model calculates change in population sizes independent of time. The Beverton & Holt (B&H) model commonly used in fish assessment includes the von Bertalanffy growth function which has age or accumulated time as an independent variable. As a result the B&H model has to assume constant fish growth. However, growth in fish is highly variable depending on food
NewWave Micromill for in-situ sampling of thin sections. Rb-Sr isochron for an Alpine blueschist. We are developing depth-profiling protocols to measure concentration profiles at sub-micron spatial resolution. The LA-ICPMS laboratory is operated by EESL The scope, installed in 2017, has x2.5, x10, x20 and x50 objectives and an AxioCam